What's Hot

    Launching survey on the future of Canada’s immigration system

    March 28, 2023

    Canada’s Team Einarson captures bronze at 2023 LGT World Women’s Curling Championship

    March 28, 2023

    OIFFA 2023 WILL TAKE PLACE FROM JUNE 13 TO 17, 2023

    March 28, 2023
    Facebook Twitter Instagram
    Facebook Twitter Instagram YouTube RSS
    Sunday, September 17
    Trending
    • Launching survey on the future of Canada’s immigration system
    • Canada’s Team Einarson captures bronze at 2023 LGT World Women’s Curling Championship
    • OIFFA 2023 WILL TAKE PLACE FROM JUNE 13 TO 17, 2023
    • Jones, Laing add to amazing curling careers with Canadian mixed doubles gold
    • Tackling the labour shortage by helping more skilled refugees and other displaced people build their careers in Canada
    • Justin Trudeau has Failed to Connect Rural Canadians
    • Through the Lenses of Science, on the Wings of Spirituality (ChakraSamhita)
    • Ra-Dha-Sva-Aah-Mi
    Bharat TimesBharat Times
    Top Banner 728 x 90 on Top
    • Home
      • Blog
      • Classes
      • Classified Bharat Times
      • Home Improvement
      • Jobs Employment Ads
      • Obituary
      • Polls
      • community
        • Community Services
        • Consulate General India
        • Montreal Communities & Temples
        • SPVM
        • Visa-Passport Bangladesh
        • Visa-Passport India
        • Visa-Passport Pakistan
        • Visa-Passport Sri-Lanka
        • High Commissioner of India in Ottawa
        • High Commissioner of Canada in India
    • Print
      • Bharat Times Newspaper (in print) Online
      • Chaimasala Newsletter
    • News
      • News
      • World
      • Editorials
      • Financial Tips
      • Indian diaspora
      • Political Interviews
      • Sports
    • Biz
      • Doing business in india
      • Classified Bharat Times
    • Life Style
      • Astrology
      • Astrologers
      • Beauty Tips
      • Health Tips
      • Kitchen
      • Spirituality
      • Travel
        • Travel Tips
      • Restaurants
    • Infotainment
      • Artists Interviews
      • Entertainment
      • Reviews
      • Cyber Security
      • Jokes
      • Games – Sudoku Online
      • Trivia
    • Events
      • Calendar
      • Upcoming Events
      • Contests
      • Bharat Times Banquets
    • Arts
      • BT Transnational Webinar Series 2021
      • Books
      • Poetry
      • Whim Whams of Journalism
    • Real Estate
      • Real Estate
    • Education
      • BT Transnational Webinar Series 2021
      • Computer Courses
      • Language Courses
      • Math & Physics Courses
      • Other Courses
      • Motivational Courses
    Bharat TimesBharat Times
    Home » Blog » Protein Stability Assay Methods
    Blog

    Protein Stability Assay Methods

    Monika SpoliaBy Monika SpoliaMarch 15, 2021Updated:September 23, 2021No Comments4 Mins Read
    Facebook Twitter LinkedIn Telegram Pinterest Tumblr Reddit WhatsApp Email
    Protein Stability Assay Methods
    Share
    Facebook Twitter LinkedIn Pinterest Email
    Homepage Top Banner 728 x 90 on Top
    Ad 12

    Are you familiar with the importance of immunoassays? These tests are of crucial value for determining the existence of antigens and antibodies in the blood of individuals.

    Since proteins play an indispensable role in detecting antibodies and antigens, it’s no wonder these are used in immunoassays. Nevertheless, determining protein stability is a complicated procedure, which has to be performed under specific conditions in order to provide accurate results. Nowadays, there are multiple assay methods, each of which offers unique benefits.

    Learn more about every technique in detail.

    DSC (Differential Scanning Calorimetry)

    One of the most popular methods for determining protein stability is Differential Scanning Calorimetry. In order to alter the natural qualities of a certain biomolecule, the technique uses heat measurement. Biomolecules with low thermal transition midpoints are believed to lack the required stability unlike those with high midpoints.

    In addition, the accurate results provided by DSC have made this analysis the most prominent when compared to other similar techniques. Hence, it’s largely used in choosing the best proteins for the creation of bio-therapeutic medicines. These drugs are based on the use of living organisms, which make protein modifications in order to deliver new treatments.

    Differential Scanning Calorimetry is believed to offer a vast array of benefits. The purpose of a protein stability assay is to determine the presence of a particular disease. Proteins are essential in the detection of antibodies and antigens, thus used in immunoassays. DSC enables a simple process of sample preparation, as well as the use of thermodynamics in the determination of parameters.

    Moreover, the samples used during the examination can be both solid and liquid, which isn’t the case in many other methods. Denaturation temperatures can also be measured by using Differential Scanning Calorimetry.

    Pulse-chase method

    The pulse-chase technique used for the purpose of determining protein stability has been popular for years in spite of the use of radioactive amino acids. It involves two stages of cellular process examination in a given time period. In the course of the first stage, cells are under exposure to a labeled compound, known as pulse. During the second stage, cells are exposed to the unlabeled form of the identical compound, known as the chase.

    The ultimate goal of the pulse-chase method is to detect the activity of given cells by monitoring their condition in a particular timeframe. It has proven surprisingly accurate in determining the half-life of proteins.

    Homepage Top Banner 728 x 90 on Top
    Top Banner 728 x 90 on Top
    Protein Stability Assay Methods - Pulse-chase method

    Due to the employment of radioactive amino acids, scientists have come up with non-radioactive versions of the same technique. Anyhow, these have never reached the popularity of the pulse-chase method, including the bleach-chase method and the cycloheximide-chase assay. See this website to learn about the mechanism and uses of a pulse-chase analysis.

    The bleach-chase technique involves using fluorophore and a light pulse in the course of bleaching. As far as the cycloheximide-chase method is concerned, cycloheximide is used instead of radioactive amino acids for the purpose of preventing synthesis.

    CD (Circular Dichroism) spectroscopy

    CD spectroscopy is widely popular in laboratories in spite of the type of information it provides. Lab technicians prefer this technique due to the small samples it requires for determining the stability of proteins.

    Additionally, the information provided by this analysis is based on the asymmetry of their structure. By detecting the absorption differences caused by structural asymmetry, CD spectroscopy offers reliable results, which lack a bit of resolution.

    Especially when it comes to taking samples from orthodontists, it becomes crucial to measure the contents for best results. This information is finally pushed to the lab, so it can easily undergo thorough research.

    Fluorescence activity assays

    Last but not least, activity assays based on fluorescence are well-known in the field of protein stability assays. Unlike the other types of analysis, this one determines the stability in an indirect manner. Nevertheless, it provides useful information about the activity and functionality of biomolecules. Check out the following link, https://www.britannica.com/science/fluorescence, for a better understanding of fluorescence.

    Nevertheless, the fluorescence activity assay isn’t always reliable in the results it provides. The use of dyes in the process of analysis has a major impact on the stability of proteins. Hence, the outcome of the analysis might not be what researchers hope for.

    The bottom line

    Regardless of the technique lab technicians use, the ultimate goal is for the stability rate to be accurate.

    It’s the only way for immunoassays to prove reliable!

     
                
     
    Protein Stability
    Share. Facebook Twitter Pinterest LinkedIn Tumblr Telegram Email
    Previous ArticleRoof Maintenance Tips Things You Need To Do To Make Your Roof Stormproof
    Next Article How to Move Your Art Works and Antiques Pieces
    Monika Spolia
    • Facebook
    • Twitter
    • Pinterest
    • Instagram
    • LinkedIn

    Related Posts

    Launching survey on the future of Canada’s immigration system

    March 28, 2023

    Canada’s Team Einarson captures bronze at 2023 LGT World Women’s Curling Championship

    March 28, 2023

    OIFFA 2023 WILL TAKE PLACE FROM JUNE 13 TO 17, 2023

    March 28, 2023
    Add A Comment

    Leave A Reply Cancel Reply

    Latest News

    Launching survey on the future of Canada’s immigration system

    March 28, 2023

    Canada’s Team Einarson captures bronze at 2023 LGT World Women’s Curling Championship

    March 28, 2023

    OIFFA 2023 WILL TAKE PLACE FROM JUNE 13 TO 17, 2023

    March 28, 2023

    Subscribe to Updates

    Get the latest sports news from SportsSite about soccer, football and tennis.

    Get new posts by email:
    Advertisement
    Demo

    Bharat Times is an intercultural newspaper (Bharat is Hindi name for India when it was inclusive of all south-Asia including Afghanistan, a couple of centuries ago). The philosophy and essence of Bharat Times is non-violence; respect of individuality and differences; tolerance leading to acceptance and grass-root spirituality – all in all Unity in Diversity.

    We're social. Connect with us:

    Facebook Twitter Instagram YouTube
    Get Informed

    Subscribe to Updates

    Get Latest news by email:
    Thoughts of Many

    I don’t believe in aging. I believe in forever altering one’s aspect to the sun. Hence my optimism.

    — Oscar Wilde, Novelist, Playwright
    © 2023 BharatTimes.ca
    • Home
    • About Us
    • ADvertise
    • Privacy Policy
    • Contributors
    • Internship
    • Volunteer
    • Contact Us
    • Sitemap

    Type above and press Enter to search. Press Esc to cancel.

    Ad Blocker Enabled!

    Ad Blocker Enabled!
    Our website is made possible by displaying online advertisements to our visitors. Please support us by disabling your Ad Blocker.